HPLC Method for Analysis of Sucralose on Primesep S2 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Sucralose · Sucralose is an synthetic organochlorine compound with the molecular formula C 12 H 19 Cl 3 O 8 . · Properties: Appearance: Typically a white, odorless, crystalline powder. · Molecular weight: ~397.6 g/mol · Solubility: Soluble in water.
Uses: An artificial sweetener used to provide sweetness in food and beverages without the calories of sugar.
Sucralose can be retained and analyzed using the Primesep S2 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and formic acid. Detection is performed using ELSD.
Method Parameters
| Mobile Phase | MeCN – 95% |
|---|---|
| Buffer | Formic Acid – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| LOD | 3.152 ppm |
| Class of Compounds | Chlorinated Disaccharide |
| Analyzing Compounds | Sucralose |
HPLC Column Used
Primesep S2, 4.6 x 150 mm, 5 µm, 100 A, dual ended