HPLC Method for Analysis of Sucralose, Mannitol, and Lactulose on Primesep S2 Column Using ELSD
https://sielc.com/hplc-method-for-analysis-of-sucralose-mannitol-and-lactuloseChromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sucralose , Mannitol , Lactulose
Sucralose is an synthetic organochlorine compound with the molecular formula C 12 H 19 Cl 3 O 8 . · Properties: Appearance: Typically a white, odorless, crystalline powder. · Molecular weight: ~397.6 g/mol · Solubility: Soluble in water.
Uses: An artificial sweetener used to provide sweetness in food and beverages without the calories of sugar.
Mannitol is an organic compound with the molecular formula C 6 H 14 O 6 .
Method Parameters
| Mobile Phase | MeCN – 85% |
|---|---|
| Buffer | Formic Acid – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Sugar |
| Analyzing Compounds | Sucralose,Mannitol,Lactulose |
HPLC Column Used
Primesep S2, 4.6 x 150 mm, 5 µm, 100 A, dual ended