HPLC Method for Analysis of Sodium Selenate on Chromni Column
https://sielc.com/hplc-method-for-analysis-of-sodium-selenateChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Sodium Selenate .
Sodium Selenate is an inorganic compound with the formula Na 2 SeO 4 . It is used primarily as an ingredient in livestock feed multivitamins to increase selenium intake. Selenium produces a red hue in glass, while Sodium Selenate is often used as a decolorizing agent. It iss also a common ingredient in pesticides.
Sodium Selenate can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using ELSD.
Method Parameters
| Mobile Phase | MeCN/H2O – 80/20 |
|---|---|
| Buffer | AmAc pH 3.0 – 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures50°C with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
| LOD* | 0.5 ppм |
| Class of Compounds | Inorganic |
| Analyzing Compounds | Sodium Selenate |
HPLC Column Used
Chromni, 4.6 x 100 mm, 3 µm, 100 A, surface coated