HPLC Method for Separation of Chloride, Selenite, Selenate, and Phosphate on Chromni Column

Chromatogram

Description

High Performance Liquid Chromatography (HPLC) Method for Analysis of Selenite , Selenate , Chloride , Sodium , Phosphate .

Chloride is typically used to refer to a compound or molecule that contains a chlorine anion: Cl – . It is an electrolyte that is essential for bodily functions including blood pH, fluid balance, cellular functions, and more. Imbalance of chloride can indicate underlying health problems.

Sodium , which  has a chemical symbol of Na , is a soft alkali metal that is highly reactive.  It is found in abundance in everyday materials like table salt, sea water, and even the Earth’s crust. It is crucial  for the body’s function and fluid balance.

Selenite , the anion, not the crystal, has the chemical formula SeO 3 2- . It’s an oxyanion of selenium.

Phosphate, PO 4 3- , is a compound that plays an important part in biological energy transfer. It contains phosphorus, which is also a key component in bones and teeth. It is also a buffer, which helps maintain pH levels in a body.

Selenate is an ion of selenium with the chemical formula SeO 3 2 . It is a good oxidizer, easily reduced to selenite and selenium.

Selenite , Selenate , Chloride , Sodium , Phosphate can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using ELSD.

Method Parameters

HPLC Column Used