HPLC Method for Analysis of Simazine on Primesep 100 Column on Cromite™
https://sielc.com/hplc-method-for-analysis-of-simazineChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Simazine
Simazine is an herbicide with the molecular formula C 7 H 12 ClN 5 . Typically, it is used to control various annual weeds in established crops. It is a pre-emergence herbicide, meaning that it has to be applied prior to the weeds emerging, as it is ineffective against grown plants.
Simazine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 50% |
|---|---|
| Buffer | Sulfuric Acid |
| Flow Rate | 0.5 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Herbicide |
| Analyzing Compounds | Simazine |
HPLC Column Used
Primesep 100, 3.2 x 100 mm, 5 µm, 100 A, dual ended