HPLC Method for Analysis of Semaglutide on Chromni Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Semaglutide .
Semaglutide is a GLP-1 receptor agonist with the chemical formula C 187 H 291 N 45 O 59 . It is used for managing type 2 diabetes and obesity. It mimics the effects of the natural hormone GLP-1, promoting insulin secretion, reducing glucagon levels, slowing gastric emptying, and suppressing appetite. These mechanisms help control blood sugar levels and support weight loss.. You can find detailed UV spectra of Semaglutide and information about its various lambda maxima by visiting the following link.
Semaglutide can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.
Method Parameters
| Mobile Phase | Gradient MeCN/H2O |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 280 nm |
| Limit Of Detection* | 103.1 ppb |
| Class of Compounds | Peptide |
| Analyzing Compounds | Semaglutide |