HPLC Method for Analysis of Ropinirole on Primesep 100 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Ropinirole .
Ropinirole is an drug with the chemical formula C 16 H 24 N 2 O . It is primarily used to treat Parkinson’s disease and restless leg syndrome. It works as a D 2 , D 3 , and D 4 dopamine receptor agonist.
Ropinirole can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 60/40 |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 195 nm |
| Limit Of Detection* | 3 ppb |
| Class of Compounds | Drug |
| Analyzing Compounds | Ropinirole |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended