HPLC – MS Method for Analysis of Rifampicin and Oxidation product on Primesep 100 Column
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Description
· HPLC Method for Analysis of Rifampicin on Primesep 100 Column by SIELC Technologies
Rifampicin is an important antibiotic with the chemical formula C 43 H 58 N 4 O 12 . It’s mainly used for the treatment of tuberculosis, often in combination with other antitubercular agents. It can also be used to treat leprosy, and as prophylaxis for Neisseria meningitidis (meningococcal) infections. It works through inhibiting bacterial DNA-dependent RNA polymerase, thereby preventing RNA synthesis and subsequent protein synthesis. You can find detailed UV spectra of Rifampicin and information about its various lambda maxima by visiting the following link.
Rifampicin can be retained, separated and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a ammonium format as a buffer. This analysis method can be detected using UV at 270 nm, an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method (CAD, ESI-MS)
Method Parameters
| Mobile Phase | MeCN – 70%, |
|---|---|
| Buffer | Ammonium Formate pH 3.0-20 mM |
| Flow Rate | 0.2 ml/min |
| Detection | UV, 270 nm, SIM 823 +, SIM 821 + |
| Spray Voltage: | 1.5 kV |
| Nebulizing gas: | 1.5 L/min |
| Drying gas: | 15 L/min |
| DL temp: | 250 ˚C |
| Heat Block: | 400 ˚C |
| Class of Compounds | Drug, antibiotics |
| Analyzing Compounds | Rifampicin |