HPLC Method for Analysis of Pyrilamine on Newcrom AH Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Pyrilamine
Pyrilamine , also known as mepyramine , is a first-generation antihistamine with the molecular formula C 17 H 23 N 3 O. It works through targeting the H1 receptor and blocking effects of histamines. It can relieve allergy symptoms like sneezing, runny nose, and itchy, watery eyes. Due to it’s ability to cross the blood-brain carrier, it might have a strong drowsiness side-effect after ingestion. When used topically, it is used to treat skin reactions to bug bites, stings, nettles rashes, and more.
Pyrilamine can be retained and analyzed using the Newcrom AH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV 310nm and LC MS ESI SIM 286.
You can find detailed UV spectra of Pyrilamine and information about its various lambda maxima by visiting the following link.
Method Parameters
| Mobile Phase | MeCN – 50% |
|---|---|
| Buffer | Ammonium Formate pH 3.0 |
| Flow Rate | 1.0 ml/min |
| Detection | UV 222 / LC MS ESI SIM 286 [M+H]+ |
| LOD*UV | 35 ppb |
| LOD* LC MS ESI SIM | 0.5 ppm |
| Class of Compounds | Antihistamine |
| Analyzing Compounds | Pyrilamine |