HPLC Method for Analysis of Pseudoephedrine on Primesep 100 Column

Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Pseudoephedrine (PSE) .

Pseudoephedrine is one of the most popular decongestant drugs on the market, in part due to its efficacy and in part due to its status as an amphetamine.  It has the chemical formula C 10 H 15 N O .  It has been reported to have been misused as a psychostimulant, since it is accessible over-the-counter.  It was a banned substance by the International Olympic Committee (IOC) until 2004, but was banned again in 2010.

Pseudoephedrine (PSE) can be separated, retained, and analyzed on a Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 200 nm with high resolution and peak symmetry.

Method Parameters

HPLC Column Used