HPLC Method for Analysis of Propanil on Newcrom B Column on Alltesta™
https://sielc.com/hplc-method-for-analysis-of-propanilChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Propanil
Propanil is a contact herbicide with the chemical formula C 9 H 9 Cl 2 NO . It is one of the most widely used herbicides in America. It works as an inhibitor of photosynthesis and CO 2 fixation in weeds.
Propanil can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 70% |
|---|---|
| Buffer | Ammonium Formate |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Herbicide |
| Analyzing Compounds | Propanil |
HPLC Column Used
Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended