HPLC Method for Analysis of Prednisolone on Newcrom CN2 Column

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Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Prednisolone · Prednisolone is an organic compound with the molecular formula C 21 H 28 O 5 . · Properties: Appearance: Typically a white to practically white, odorless, crystalline powder. · Molecular weight: ~360.4 g/mol · Solubility: Soluble in organic solvents like methanol, dioxane, and ethanol.

Uses: A widely used corticosteroid medication that provides relief from various health conditions by reducing inflammation and suppressing the immune system.

Prednisolone can be retained and analyzed using the Newcrom CN2 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and phosphoric acid. Detection is performed using UV at 240 nm.

Method Parameters

Mobile PhaseMeCN – 10%
BufferH3PO4 – 0.1%
Flow Rate0.5 ml/min
DetectionUV 240 nm
Class of CompoundsCorticosteroid
Analyzing CompoundsPrednisolone

HPLC Column Used

Newcrom CN2, 3.2 x 100 mm, 5 µm, 100 A, dual ended
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