HPLC Method for Analysis of Potassium Sorbate on Newcrom BH Column
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Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Potassium sorbate , Sorbic acid .
Potassium Sorbate is a popular preservative with the chemical formula C 6 H 7 K O 2 . It is used in a wide variety of foods, wines, and personal-care products. It works through inhibiting molds, yeasts, and various other microbes. You can find detailed UV spectra of Potassium Sorbate and information about its various lambda maxima by visiting the following link.
Potassium sorbate , Sorbic acid can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 20% |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250 nm |
| Class of Compounds | Salt |
| Analyzing Compounds | Potassium sorbate,Sorbic acid |
HPLC Column Used
Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended