HPLC MS Method for Analysis of Phytic Acid on BIST A Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Phytic Acid
Phytic Acid , also known as inositol hexaphosphate , inositol hexakisphosphate ( IP6 ), AND inositol polyphosphate is a dihydrogenphosphate ester of inositol with the molecular formula C 6 H 18 O 24 P 6 . It is also known as a phosphorus-storage compounds primarily found in nuts, seeds, and whole grains. It serves as a way for plants to store phosphorus. When broken down in the body, it binds to chelates.
Phytic Acid can be retained and analyzed using the BIST A stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.
Method Parameters
| Mobile Phase | Gradient MeCN – 60/40% – 10/90%, 10 min |
|---|---|
| Buffer | TMEDA formate |
| Flow Rate | 0.2 mL/min |
| Detection | LC MS ESI SIM |
| LOD*UV | 2 ppm |
| Class of Compounds | Ester |
| Analyzing Compounds | Phytic Acid |
HPLC Column Used
BIST A, 2.1 x 50 mm, 5 µm, 100 A, dual ended