HPLC Method for Analysis of Phenazopyridine in Pharmaceutical Formulation on Primesep 100 Column
https://sielc.com/hplc-method-for-analysis-of-phenazopyridineChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Phenazopyridine .
Phenazopyridine is an drug with the chemical formula C 11 H 11 N 5 . It is has a local analgesic effect on the urinary tract, therefore it is often used to help with pain, irritation, and urgency caused by urinary tract infections, injury, or surgery. It is not yet known exactly how the drug works.
Phenazopyridine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 70/30 |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 390nm |
| Limit Of Detection* | 80 ppb |
| Class of Compounds | Drug |
| Analyzing Compounds | Phenazopyridine |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended