HPLC Method for Analysis of Pentosidine
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Pentosidine
Pentosidine is a advanced glycation end-product (AGE) with C 17 H 26 N 6 O 4 • CF 3 COOH chemical formula. Since it is a fluorescent biomarker, it is often used as an indicator in studies of aging and chronic diseases including diabetes and uremia.
Pentosidine can be retained and analyzed using the Obelisc R stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium acetate buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 20% |
|---|---|
| Buffer | Ammonium acetate |
| Flow Rate | 1.0 ml/min |
| Detection | UV |
| Class of Compounds | Advanced Glycation End-Product |
| Analyzing Compounds | Pentosidine |
HPLC Column Used
Obelisc R, 4.6 x 150 mm, 5 µm, 100 A, dual ended