HPLC Method For Analysis Of Patulin on Newcrom R1 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Patulin.
Patulin is a toxic chemical contaminant produced by several species of mold, especially within Byssochlamys Aspergillus and Penicillium. It is the most common mycotoxin found in apples and apple-derived products such as juice, cider, compotes and other food intended for young children.
Patulin can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN, ACN), water and Phosphoric Acid (H3PO4) buffer. The analysis method can be UV detected at 275 nm.
Method Parameters
| Mobile Phase | MeCN – 10% |
|---|---|
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 0.5 ml/min |
| UV Detection | 275 nm |
| LOD | 0.002 ppm |
| Class of Compounds | Hydrophilic |
| Analyzing Compounds | Patulin |
HPLC Column Used
Newcrom R1, 3.2 x 100 mm, 5 µm, 100 A, dual ended