HPLC Method for Analysis of Pantoprazole Sodium in Tablets on Newcrom R1
https://sielc.com/hplc-method-for-analysis-of-pantoprazole-sodium-in-tabletsChromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Pantoprazole , Pantoprazole sodium
Pantoprazole is a proton pump inhibitor (PPI) with C 16 H 15 F 2 N 3 O 4 S molecular formula. It is used to decrease stomach acids amounts. It does that through binding to proton pumps in the stomach’s lining, therefore preventing the production of stomach acid.
Pantoprazole , Pantoprazole sodium can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Triethanolamine buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 25/75% |
|---|---|
| Buffer | TEAPh – 10 mM |
| Flow Rate | 1.0ml/min |
| Detection | UV, 290 nm |
| Class of Compounds | Drug, Proton-pump inhibitor, Hydrophobic, Neutral |
| Analyzing Compounds | Pantoprazole,Pantoprazole sodium |
HPLC Column Used
Newcrom R1, 4.6 x 100 mm, 5 µm, 100 A, dual ended