HPLC Method for Analysis of Oligonucleotide 5A on Chromni™ Column on Alltesta™
https://sielc.com/hplc-method-for-analysis-of-oligonucleotide-5aChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Oligonucleotide 5A.
Oligonucleotide 5A is a small RNA molecule with a specific adenine sequence. They are primarily used to inhibit viral and cancer cell growth. In RNA synthesis, they are intermediates in enzymatic synthesis of capped RNAs.
Oligonucleotide 5A can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | Gradient MeCN – 80-60% |
|---|---|
| Buffer | Ammonium Formate |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Nucleotide Base |
| Analyzing Compounds | Oligonucleotides |
HPLC Column Used
Chromni, 4.6 x 150 mm, 5 µm, 100 A, dual ended