HPLC Method for Analysis of Oligonucleotides dt 5 mer on BIST A Column
https://sielc.com/hplc-method-for-analysis-of-oligo5tChromatogram
Description
· Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies · HPLC Method for Analysis of DNA Oligo, 5 bases (TT TTT) on BIST A Column by SIELC Technologies
DNA oligos, short for oligonucleotides, are small fragments of DNA that are often synthesized for various scientific and medical applications. A DNA oligo with a sequence of 5 thymine bases, denoted as “TTTTT,” has some specific properties and uses:
Composition : This oligo is composed of five thymine (T) nucleotides, which are pyrimidine bases.
Stability : Thymine-rich oligos can have different stability characteristics compared to those rich in other nucleotides. The stability of DNA strands is influenced by base stacking interactions and hydrogen bonding.
Hybridization Potential : The ability of this oligo to hybridize (bind) with other DNA or RNA sequences is limited due to its homopolymer nature. It would preferentially bind to sequences rich in adenine (A).
DNA oligo with the sequence “TTTTT” has unique properties related to its homopolymer nature and finds specific uses in molecular biology, although it also has some limitations due to its repetitive sequence.
Method Parameters
| Mobile Phase | MeCN – 60% |
|---|---|
| Buffer | TMEDA Formate pH 4.0 – 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260 nm |
| Sample | 0.024 mg/ml in EtOH/H2O – 50/50% |
| Injection volume | 1 µl |
| LOD* | 10 ppb |
| Class of Compounds | Oligonucleotides |
| Analyzing Compounds | Oligonucleotides |