HPLC Method for Analysis of Nirmatrelvir in PAXLOVID™ (Nirmatrelvir Tablets) on Primesep P Column
https://sielc.com/hplc-method-for-analysis-of-nirmatrelvir-in-paxlovid-nirmatrelvir-tabletsChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Nirmatrelvir .
Nirmatrelvir is an antiviral medication with the chemical formula C 23 H 32 F 3 N 5 O 4 . It is a part of a medical combination used to treat COVID-19. It works through inhibiting 3C-like protease. From November 2021, Nirmatrelvir has been allowed to be manufactured and sold in over 95 countries to ensure greater access to the global population.
Nirmatrelvir can be retained and analyzed using the Primesep P stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-90% |
|---|---|
| Buffer | No |
| Flow Rate | 0.5 ml/min |
| Detection | UV 210nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Nirmatrelvir |
HPLC Column Used
Primesep P, 3.2 x 100 mm, 5 µm, 100 A, dual ended