HPLC Method for Analysis of Nirmatrelvir and Ritonavir in Paxlovid Tablets on Primesep 100 Column

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Chromatogram

Chromatogram

Description

· Separation type: Liquid Chromatography Mixed-mode · High Performance Liquid Chromatography (HPLC) Method for Analysis of Nirmatrelvir and Ritonavir

Method Parameters

Mobile PhaseGradient MeCN – 50-70%
BufferH2SO4 – 0.5%
Flow Rate0.5 ml/min
DetectionUV 210nm
Class of CompoundsDrug
Analyzing CompoundsNirmatrelvir,  Ritonavir

HPLC Column Used

Promesep 100, 3.2×100 mm,  100A