HPLC Method for Analysis of Nicotinamide on Primesep 100 Column on Alltesta™
https://sielc.com/hplc-method-for-analysis-of-nicotinamideChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Nicotinamide
Nicotinamide , also known as niacinaminde , is a form of vitamin B 3 with the chemical formula C 6 H 6 N 2 O 2 . It is preferred treatment for pellagra and is a common treatment for acne due to it’s anti-inflammatory properties. If consumed daily, it is shown to decrease the risk of skin cancers other than melanoma.
Nicotinamide can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 50% |
|---|---|
| Buffer | Sulfuric Acid |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Vitamin |
| Analyzing Compounds | Nicotinamide |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended