HPLC Method for Analysis of Neostigmine methylsulfate on Primesep 100 Column
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Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Neostigmine methylsulfate , Norneostigmine .
Neostigmine methylsulfate is a drug with the chemical formula C 13 H 22 N 2 O 6 S . It is primarily used to reverse effects of non-depolarizing neuromuscular blocking agents after surgery. It works through inhibiting the enzyme acetylcholinesterase, preventing the breakdown of the neurotransmitter acetylcholine, which leads to a buildup of it at nerve endings, which stimulates muscle contractions.
Neostigmine methylsulfate , Norneostigmine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 30% |
|---|---|
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Neostigmine methylsulfate,Norneostigmine |