HPLC Method for Analysis of Neopterin on Primesep 100 Column on Alltesta™
https://sielc.com/hplc-method-for-analysis-of-neopterinChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Neopterin
Neopterin is an pteridine with the chemical formula C 9 H 11 N 5 O 4 . It is indicative of a pro-inflammatory immune status, therefore it typically serves as a marker of the activation of the cellular immune system.
Neopterin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 5% |
|---|---|
| Buffer | Ammonium Formate |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Organic Compound |
| Analyzing Compounds | Neopterin |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended