HPLC Method for Analysis of Myelin Basic Protein (85-99) Peptide Antagonist on BIST B+ Column
https://sielc.com/hplc-method-for-analysis-of-myelin-basic-protein-85-99-peptide-antagonistChromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Myelin Basic Protein Peptide Antagonist
Myelin Basic Protein Peptide Antagonist is a synthetic peptide antagonist with the molecular formula C 70 H 114 N 18 O 21 • XCF 3 COOH . When expressing an MBP (85-99), it inhibits the production of IL-2 in T cell hybridoma cells. MBP peptide antagonist also delays MBP (85-99)-induced experimental autoimmune encephalomyelitis and reduces symptom severity.
Myelin Basic Protein Peptide Antagonist can be retained and analyzed using the BIST B+ stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.
Method Parameters
| Mobile Phase | Gradient MeCN – 70/30% – 50/50%, 10 min |
|---|---|
| Buffer | Sulfuric Acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 |
| LOD*UV | 194 ppb |
| Class of Compounds | Peptide Antagonist |
| Analyzing Compounds | Myelin Basic Protein Peptide Antagonist |
HPLC Column Used
BIST B+, 4.6 x 50 mm, 5 µm, 100 A, dual ended