Low-UV Detection HPLC Method for PEI Analysis on PEI Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Polyethylenimine PEI
Polyethylenimine ( PEI ), also known as polyaziridine , is a polymer with the molecular formula (C 2 H 5 N) n . It has repeating units of the amine group and carbon aliphatic spacers. PEI has a large variety of uses. Most commonly, it is used in detergents, adhesives, water treatment agents, and cosmetics. Medically, due to being a catatonic polymer, it has been also studied as a gene delivery vehicle with successful outcomes.
Polyethylenimine PEI can be retained and analyzed using the PEI stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.
Method Parameters
| Mobile Phase | 0.05% HClO4in MeCN/H2O – 20/80% |
|---|---|
| Flow Rate | 0.5 mL/min |
| Detection | UV 200 |
| LOD*UV | 60 ppb |
| Class of Compounds | Polymer |
| Analyzing Compounds | Polyethylenimine PEI |