HPLC Method For Analysis Of Minocycline on Primesep 100 column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Minocycline hydrochloride
Minocycline hydrochloride is a tetracycline antibiotic with C 23 H 27 N 3 O 7 • HCl [XH 2 O] molecular formula. It is used to treat a wide variety of bacterial infections, acne, and sexually transmitted infections. Despite that, it is generally less preferred as a medication to tetracycline doxycycline.
Minocycline can be retained on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 270 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50% |
|---|---|
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Antibiotics |
| Analyzing Compounds | Minocycline hydrochloride |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended