HPLC Method for Analysis of Metronidazole on Newcrom R1 Column on Alltesta™
https://sielc.com/hplc-method-for-analysis-of-metronidazole-2Chromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Metronidazole
Metronidazole is an antibiotic with C 6 H 9 N 3 O 3 molecular formula. It works strongly against anaerobic bacteria and protozoa. It is available as oral tablets, capsules, topical gels, and injections. To truly kill bacteria and prevent infection, a full course of the medication must be completed as prescribed by a doctor.
Metronidazole can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 5% |
|---|---|
| Buffer | H3PO4 – 0.21 |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Antibiotic |
| Analyzing Compounds | Metronidazole |
HPLC Column Used
Newcrom R1, 4.6 x 150 mm, 5 µm, 100 A, dual ended