HPLC Method for Analysis of Metoprolol on Primesep 200 Column on Alltesta™
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Metoprolol
Metoprolol is a medication with the chemical formula C 15 H 25 NO 3 . It is primarily used to treat angina, high blood pressure, abnormally fast heart rate, and to prevent heart problems after a myocardial infraction. It works through blocking β1-adrenergic receptors, which increase heartrate.
Metoprolol can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 50% |
|---|---|
| Buffer | Phosphoric Acid |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Metoprolol |
HPLC Column Used
Primesep 200, 4.6 x 150 mm, 5 µm, 100 A, dual ended