HPLC Method for Analysis of Metoprolol in Tablets on Primesep 200 Column
https://sielc.com/hplc-method-for-analysis-of-metoprolol-in-tabletsChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Metoprolol
Metoprolol is a beta-blocker that is used to treat high blood pressure, angina, and heart failure by blocking beta-1 adrenergic receptors. At it’s most effective, it can lower the risk of death after a heart attack.
Metoprolol can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50% |
|---|---|
| Buffer | H3PO4 – 0.5 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 223 nm |
| Class of Compounds | Drug, Beta-blocker, Hydrophobic, Basic |
| Analyzing Compounds | Metoprolol |
HPLC Column Used
Primesep 200, 4.6 x 150 mm, 5 µm, 100 A, dual ended