HPLC Method for Analysis of Metalaxyl on Newcrom B Column on Alltesta™
https://sielc.com/hplc-method-for-analysis-of-metalaxylChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Metalaxyl
Metalaxyl , also known as methyl N-(methoxyacetyl)-N-(2,6-xylyl)-DL-alaninate, is a systemic fungicide with the chemical formula C 15 H 21 NO 4 . It is used to control Pythium and Phytophthora in vegetables. It has suffered a resistance problem going back to the 1980s.
Metalaxyl can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 50% |
|---|---|
| Buffer | Ammonium Formate |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Pesticide |
| Analyzing Compounds | Metalaxyl |
HPLC Column Used
Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended