HPLC Method for Analysis of Mannitol on Primesep S2 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Mannitol · Mannitol is an organic compound with the molecular formula C 6 H 14 O 6 . · Properties: Appearance: Typically a white, odorless, crystalline powder or granules. · Molecular weight: ~182.17 g/mol · Solubility: Soluble in water. · Uses: A versatile medication with various uses in healthcare, primarily as an osmotic diuretic.
Mannitol can be retained and analyzed using the Primesep S2 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and formic acid. Detection is performed using ELSD.
Method Parameters
| Mobile Phase | MeCN – 85% |
|---|---|
| Buffer | Formic Acid – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| LOD | 4.380 ppm |
| Class of Compounds | Sugar Alcohol |
| Analyzing Compounds | Mannitol |
HPLC Column Used
Primesep S2, 4.6 x 150 mm, 5 µm, 100 A, dual ended