HPLC Method for Analysis of Lycopene and beta-Carotene on Lipak Column on Cromite™

Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of beta-Carotene , Lycopene .

Lycopene is a carotenoid hydrocarbon with C 40 H 56 molecular formula. It is found primarily in tomatoes, watermelon, grapefruits, and red guavas. Gac has the highest concentration of lycopene at 2~6 per gram. Lycopene in non-toxic, but can be a rare allergen. You can find detailed UV spectra of Lycopene and information about its various lambda maxima by visiting the following link.

beta-Carotene , also written as β-Carotene , is a pigment with the chemical formula C 40 H 56 . It is found primarily in fungi, plants, and fruit. Especially high amounts of it are found in carrots, pumpkins, spinach, and sweet potatoes. In dietary contexts, it is used as a pro-vitamin, as it converts to retinol in the body. In fact, it is the worldwide primary dietary source of provitamin A and is the best known provitamin a carotenoid. You can find detailed UV spectra of beta-Carotene and information about its various lambda maxima by visiting the following link.

beta-Carotene , Lycopene can be retained and analyzed using the Lipak stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) and ethanol. Detection is performed using Vis.

Method Parameters

HPLC Column Used