HPLC UV Method for Analysis of Natamycin in Yogurt on Primesep 100 Column
https://sielc.com/hplc-method-for-analysis-of-lnatamycin-in-yogurtChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Natamycin .
Natamycin , also known as pimaricin , is an antifungal medication with C 33 H 47 NO 13 molecular formula. It is primarily used to treat fungal infections of eyelids, conjunctiva, and cornea. On occasion, it is also used as a preservative. It prevents fungi growth by binding to ergosterol in fungal cell membranes and inhibiting amino acid and glucose transport proteins. You can find detailed UV spectra of Natamycin and information about its various lambda maxima by visiting the following link.
**The Yogurt sample was prepared by mixing 5g of yogurt and 5g or acetonitrile. After the mixture separated, the liquid half was removed and filtered.
Natamycin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) and ethanol. Detection is performed using UV.
Method Parameters
| Mobile Phase | Gradient MeCN/H2O 10/90 to 80/20% over 20 min |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 320 nm |
| Limit Of Detection* | Natamycin – 0.2 ppb |
| Class of Compounds | Drug |
| Analyzing Compounds | Natamycin |