HPLC Method for Analysis of Lamivudine on Primesep 100 Column on Alltesta™
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Lamivudine
Lamivudine is an antiviral medication with the chemical formula C 8 H 11 N 3 O 3 S . It is used primarily to treat infections caused by the human immunodeficiency virus (HIV) and hepatitis B virus (HBV). It belongs to a class of drugs known as nucleoside reverse transcriptase inhibitors (NRTIs). You can find detailed UV spectra of Lamivudine and information about its various lambda maxima by visiting the following link.
Lamivudine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN -50/50% |
|---|---|
| Buffer | AmFm pH 3.0 – 20 mM |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Medication |
| Analyzing Compounds | Lamivudine |