HPLC Method for Analysis of Lactulose on Primesep S2 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Lactulose · Lactulose is an synthetic disaccharide with the molecular formula C 12 H 22 O 11 . · Properties: Appearance: Typically a white, odorless crystalline powder with a sweet taste. · Molecular weight: ~342.30 g/mol · Solubility: Soluble in water. · Uses: Used for treating constipation and hepatic encephalopathy (HE).
Lactulose can be retained and analyzed using the Primesep S2 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and formic acid. Detection is performed using ELSD.
Method Parameters
| Mobile Phase | MeCN – 80% |
|---|---|
| Buffer | Formic Acid – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| LOD | 7.643 ppm |
| Class of Compounds | Synthetic Disaccharide |
| Analyzing Compounds | Lactulose |
HPLC Column Used
Primesep S2, 4.6 x 150 mm, 5 µm, 100 A, dual ended