HPLC Method For Analysis Of L-Carnitine and Arginine on Primesep 100 Column
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Description
· Separation type: Liquid Chromatography Mixed-mode · High Performance Liquid Chromatography (HPLC) Method for Analysis of L-Carnitine and Arginine
L-carnitine is a quaternary ammonium cation (also known as a quat) that is key to the metabolic processes of most living species. Arginine is an alpha amino acid that is key to the biosynthesis of proteins. These two amine-based compounds can be detected in the low UV regime. Using a Primesep 100 mixed-mode column and a gradient mobile phase consisting of water and acetonitrile (MeCN) with a gradient of sulfuric acid (H2SO4) as a buffer, L-carnitine and Arginine can be retained, separated, and analyzed. This analysis method can be UV detected at 205 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | Gradient H2SO4 from 0.1 to 0.5%, 10 min |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 205 nm |
| Class of Compounds | Amino acid |
| Analyzing Compounds | Carnitine, Arginine |
HPLC Column Used
Primesep 100, 4.6×150 mm, 5 µm, 100A