HPLC Method For Analysis of Ivermectin on Primesep 100 column
https://sielc.com/hplc-method-for-analysis-of-ivermectinChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Ivermectin B1a .
Ivermectin is an anti-parasitic drug with the chemical formula C 48 H 74 O 14 . It is often used to treat or prevent parasites in animals and humans. In dogs, it is routinely used to treat heartworm, though, certain breeds, can be severely poisoned by this medication. You can find detailed UV spectra of Ivermectin B1a and information about its various lambda maxima by visiting the following link.
Ivermectin can be retained and separated on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN), and sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 250 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 60/40% |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds | Drug, Antibiotics |
| Analyzing Compounds | Ivermectin B1a |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended