HPLC Method for Analysis of Isonicotinic Acid on Primesep 100 Column on Alltesta™
https://sielc.com/hplc-method-for-analysis-of-isonicotinic-acidChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Isonicotinic Acid
Isonicotinic Acid is an nucleoside with the C 6 H 5 NO 2 molecular formula. It is a derivative of pyridine, making it a pyridinemonocarboxylic acid. It acts primarily as a metabolite in humans and algae.
Isonicotinic Acid can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 30% |
|---|---|
| Buffer | H2SO4– 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Isonicotinic Acid |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended