HPLC UV Method for Analysis of Imetit on Chromni Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Imetit .
Imetit is a histamine H 3 receptor agonist with the chemical formula C 6 H 10 N 4 S . You can find detailed UV spectra of Imetit and information about its various lambda maxima by visiting the following link.
Imetit can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 80% |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 |
| Limit Of Detection* | 30 ppb |
| Class of Compounds | Histamine Receptor Agonist |
| Analyzing Compounds | Imetit |
HPLC Column Used
Chromni, 4.6 x 150 mm, 3 µm, 100 A, dual ended