HPLC UV Method for Analysis of Imetit on Primesep A Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Imetit .
Imetit is a histamine H 3 receptor agonist with the chemical formula C 6 H 10 N 4 S . You can find detailed UV spectra of Imetit and information about its various lambda maxima by visiting the following link.
Imetit can be retained and analyzed using the Primesep A stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 80% |
|---|---|
| Buffer | H2SO4 – 0.4% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 |
| Limit Of Detection* | 130 ppb |
| Class of Compounds | Histamine Receptor Agonist |
| Analyzing Compounds | Imetit |
HPLC Column Used
Primesep A, 4.6 x 150 mm, 5 µm, 100 A, dual ended