HPLC Method for Analysis of Glycine Methyl Ester Hydrochloride
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Glycine methyl ester , Glycine
Glycine Methyl Ester Hydrochloride is a water soluble organic compound that is typically used in synthesis of pharmaceuticals and pesticides. Besides that, it is also used as a flavor enhancer and nutritional supplement.
Glycine Methyl Ester Hydrochloride can be retained and analyzed using the Primesep-A stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 10/90% |
|---|---|
| Buffer | H2SO4 – 0.05% |
| Flow Rate | 0.05 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds | Amino acid, Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Zwitterionic |
| Analyzing Compounds | Glycine methyl ester,Glycine |
HPLC Column Used
Primesep A, 3.2 x 150 mm, 5 µm, 100 A, dual ended