HPLC Method for Analysis of Glycerol on Primesep AP Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Glycerol · Glycerol is an organic compound with the molecular formula C 3 H 8 O 3 . · Properties: Appearance: Typically a colorless, odorless, and syrupy liquid. · Molecular weight: ~92.09 g/mol · Solubility: Soluble in Water.
Uses: Used in many industries and for many products including skin care, sweetener, dry skin solutions, soap, moisturizers, pharmaceuticals, humectant and food.
Glycerol can be retained and analyzed using the Primesep AP stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and formic acid. Detection is performed using ELSD or RI.
Method Parameters
| Mobile Phase | MeCN – 90% |
|---|---|
| Buffer | Formic Acid – 0.5% |
| Detection | ELSD or RI |
| Class of Compounds | Simple Triol |
| Analyzing Compounds | Glycerol |
HPLC Column Used
Primesep AP, 4.6 x 150 mm, 5 µm, 100 A, dual ended