LCMS Method for Analysis of N-Acetylglucosamine-6-phosphate and N-Acetylglucosamine-1-phosphate on Newcrom B Column
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Description
HPLC Method for Analysis of N-Acetylglucosamine-1-phosphate , N-Acetylglucosamine-6-phosphate on Newcrom B Column by SIELC Technologies
N-Acetylglucosamine-6-phosphate (GlcNAc-6-P) and N-Acetylglucosamine-1-phosphate (GlcNAc-1-P) are two phosphorylated derivatives of N-acetylglucosamine (GlcNAc), a monosaccharide that is part of various biological structures, such as glycoproteins and glycosaminoglycans.
Both GlcNAc-6-P and GlcNAc-1-P are involved in the regulation of metabolism and glycosylation, but they differ in the position of the phosphate group on the glucose structure.
N-Acetylglucosamine-6-phosphate (GlcNAc-6-P) and N-Acetylglucosamine-1-phosphate (GlcNAc-1-P) can be retained, separated and analyzed using an Newcrom B mixed-mode stationary phase column. The analysis employs a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and formic acid as a buffer. Detection is achieved using LC MS.
Method Parameters
| Mobile Phase | MeCN/H2O – 60/40 % |
|---|---|
| Buffer | Formic Acid – 1.0% |
| Flow Rate | 0.5 ml/min |
| Detection | SIM 300 -, SIM 302 + |
| Sample | 0.5 mg/ml |
| Injection volume | 1 µl |
| LOD* | 300 ppb |
| Class of Compounds | Amino sugars |
| Analyzing Compounds | N-Acetylglucosamine-1-phosphate,N-Acetylglucosamine-6-phosphate |