HPLC MS Method for Analysis of Copper Peptide GHK-Cu on Primesep 200 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Copper peptide (GHK-Cu)
Copper Peptide GHK-Cu is a naturally occurring copper complex with the molecular formula C14H 22 CuN 6 O 4 • C 2 H 4 O 2 . Research shows that the complex facilitates faster wound healing, improved angiognesis, and elevated level of antioxidant enzymes. Often times, it is used in anti-aging cosmetics.
Copper peptide (GHK-Cu) can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using LC MS.
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Method Parameters
| Mobile Phase | MeCN – 20% |
|---|---|
| Buffer | Ammonium formate |
| Flow Rate | 0.5 ml/min |
| Detection | LC MS ESI GHK (free peptide, [M+H]+) 341 |
| Class of Compounds | Complex |
| Analyzing Compounds | Copper peptide (GHK-Cu) |