HPLC UV Method for Analysis of Gallic Acid in Bismuth Subgallate Tablets (Devrom) on Newcrom BH Column
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Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Bismuth subgallate , Gallic acid .
Gallic acid is a naturally-occurring phenolic acid with the chemical formula the chemical formula C 7 H 6 O 5 . It can be found in gallnuts, sumac, witch hazel, tea leaves, oak bark, and other plants. Historically, it was a key ingredient in European iron gall ink. The use of it spanned from its first discovery in the 12th century into the 19th century. In the modern times, it is used as an antioxidant and a preservative. You can find detailed UV spectra of Gallic acid and information about its various lambda maxima by visiting the following link.
Bismuth subgallate is a medication with the chemical formula C 7 H 5 BiO 6 . It is primarily used to deodorize flatulence and stools. Additionally, it is used to treat the Helicobacter pylori infection.
Bismuth subgallate , Gallic acid can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 10% |
|---|---|
| Buffer | H3PO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 273nm |
| Class of Compounds | Acid, Hydrophilic |
| Analyzing Compounds | Bismuth subgallate,Gallic acid |