HPLC Method for Analysis of Gabapentin Tablets
https://sielc.com/hplc-method-for-analysis-of-gabapentin-tabletsChromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Gabapentin , Gabapentin hydrochloride
Gabapentin is a drug used to treat epilepsy, neuropathic pain, hot flashes, and restless legs syndrome. It also is used to treat neuropathic pain. Gabapentin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It is also suitable for pharmacokinetics.
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50% |
|---|---|
| Buffer | H2SO4 – 0.05 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds | Drug, Nerve pain medication, Anticonvulsant, Hydrophobic, Ionizable, Zwitterionic |
| Analyzing Compounds | Gabapentin,Gabapentin hydrochloride |