HPLC Method for Analysis of Formic Acid and Acetic Acid on Chromni Column

Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Formic acid , Acetic Acid .

Formic Acid is the simplest carboxylic acid with the chemical formula CH 2 O 2 . It is naturally found in insects, weeds, fruits, and vegetables. It is used by insects as a method of self-defense. In agriculture, it is used as a preservative and antibacterial agent. In chromatography, it is used as a volatile pH modifier. It is used significantly in the tanning of leather, dyeing and finishing of textiles, and production of rubber. You can find detailed UV spectra of Formic Acid and information about its various lambda maxima by visiting the following link.

Acetic Acid is a simple carboxylic acid with the formula CH 3 COOH . It’s an important chemical reagent and industrial chemical, primarily used in the production of cellulose acetate for photographic film and polyvinyl acetate for wood glue, as well as synthetic fibers and fabrics. It gives vinegar its sour taste and pungent smell. It is well known for being the active ingredient in vinegar, leading to the belief that it is the earliest mass produced acid, dating back to 3BC.

Formic acid , Acetic Acid can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.

Method Parameters

HPLC Column Used