HPLC Method for Analysis of Fluconazole on Primesep B Column
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Description
Fluconazole is an antifungal medication used to treat and prevent various fungal infections. Here are some key points about fluconazole:
1. Mechanism of Action: · 2. Indications (Uses):
Fluconazole can be retained, and analyzed on a Primesep B mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a ammonium format a buffer. This analysis method can be detected in the UV 270 nm.
Method Parameters
| Mobile Phase | MeCN – 20% |
|---|---|
| Buffer | Ammonium Formate pH 4.0-20 mM |
| Flow Rate | 0.2 ml/min |
| Detection | UV, 250 nm |
| Peak Retention Time | 3.97 min |
| Class of Compounds | Allylamines |
| Analyzing Compounds | Fluconazole |
HPLC Column Used
(Column variation not found)